Skip to Main Content
Adobestock 375639711

Biologics and Antibodies

Biologics and Antibodies

In addition to laboratory services, rtilab produces a variety of monoclonal antibodies, peripheral blood mononuclear cells (PBMCs) and pulmonary alveolar macrophage (PAM) cells for purchase. 

Please see our catalog below for current listings.

Rtilab MAb Products

HRP conjugations available upon request. Please inquire.

Rtilab PAMs Cells

Products Descriptions

Bovine Coronavirus (BCV) Monoclonal Antibody (BC 6-4)  ♦  BC 6-4 reacts with North American strains of Bovine Coronavirus (BCV). This monoclonal antibody recognizes an epitope on the nucleocapsid protein of BCV.  The antibody is produced as mouse ascites fluid. One BC 6-4 product is available: BC 6-4-A (unconjugated ascites fluid).  Isotype-IgG1. Uses of BC 6-4-IHC, IFA, ELISA

Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Monoclonal Antibody (AT13-A) ♦ Monoclonal antibody AT-13 recognizes a highly conserved, conformational dependent epitope within the nucleocapsid protein of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). It was produced against a European-like strain (U.S. type I) recently isolated within the United States. It has a high degree of cross reactivity to European, European­ like, and North American isolates of PRRSV. This monoclonal antibody is produced as  mouse ascites fluid. One AT13 product is available: AT13-A (unconjugated ascites fluid).  Isotype: IgM. Product AT-13 is recommended for use in immunoprecipitation, indirect fluorescent antibody, and enzyme linked-immunosorbent assay applications. AT-13 does not recognize proteins on Western blot.

Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Monoclonal Antibody (SDOW17)  ♦  SDOW17 reacts with >99% of North American and European isolates of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). This monoclonal antibody recognizes a highly conserved epitope on the nucleocapsid protein of PRRSV. SDOW17 does not react with the Prime Pac PRRSV vaccine strain. This antibody is produced as mouse ascites fluid. Two SDOW17 products are available: SDOW17-A (unconjugated ascites fluid) and SDOW17-F (ascites fluid conjugated with fluorescein isothiocyanate {FITC}).  Isotype-IgG1. Uses and Recommended Dilutions - SDOW17-A: IFA—1:50,000, IHC—1:10,000. SDOW17-F: IFA—1:100

Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Monoclonal Antibody (SR30)  ♦  SR30 reacts with essentially all North American and European strains of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). This monoclonal antibody recognizes a highly conserved epitope on the nucleocapsid protein of PRRSV. The antibody is produced as mouse ascites fluid. Two SR30 products are available: SR30-A (unconjugated ascites fluid) and SR30-F (ascites fluid conjugated with fluorescein isothiocyanate {FITC}). Isotype-IgG1. Uses and Recommended Dilutions - SR30-A:  IFA—1:50,000, IHC—1:10,000, SR30-F: IFA—1:100

Escherichia coli K88ac Monoclonal Antibody (EC K88)  ♦  The anti-K88ac monoclonal antibody was created against the K88 fimbriae of enterotoxigenic Escherichia coli (ETEC). The antibody reacts with K88 fimbriae of ETEC and blocks the binding of K88+ bacteria to intestinal epithelial cells. The antibody is produced in hybridoma cells and is available as cell culture supernatant. Isotype-IgG1. Uses of EC K88-IFA. Recommended Dilution—1:100

Rotavirus (RV) Monoclonal Antibody (RV 11-2)  ♦  RV 11-2 reacts with Group A strains of rotavirus (RV). This monoclonal antibody recognizes an epitope on the VP6 protein of RV. The antibody is produced as mouse ascites fluid. One RV 11-2 product is available: RV 11-2-A (unconjugated ascites fluid). Isotype-IgG2a. Uses of RV 11-2: RV11-2-A: IFA, ELISA. 

Porcine Circovirus Monoclonal Antibody (PCV2)  ♦  PCV2 reacts with porcine circovirus 2 virus. The antibody is produced as mouse ascites fluid. One PCV2 product is available: PCV2-A (unconjugated ascites fluid). Isotype-IgG2. Uses and Recommended Dilution, IFA—1:1000

Conjugation Information (Please Inquire for Availability)

Antibodies can be conjugated to different labels, including enzymes, biotin, and fluorescent dyes/proteins. The label of choice essentially depends upon the experimental application.

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light, causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue, or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques because the conjugation of FITC to proteins is relatively easy and generally does not destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Antibodies to FITC are used to identify FITC labeled proteins and as models to study the mechanism of antibody response to a well-defined hapten.

Biotin is commonly used when the target of interest is expressed at low levels and cannot be detected using secondary labeled antibodies alone. Many biotin molecules can be conjugated to a secondary antibody.

Enzymes are proteins, and the molecules upon which they act are known as substrates. The enzyme label can be visualized by means of enzyme histochemical methods via chromogenic reactions, wherein a soluble colorless substrate is converted to a water-insoluble colored compound. One of the more commonly used enzymes is Horse radish peroxidase (HRP).

Horse radish peroxidase (HRP) can be visualized by chromogenic reactions such as chemiluminescence or through the use of diaminobenzidine (DAB). HRP is a 44kDa glycoprotein enzyme label and is more stable than alkaline phosphatase.

Contact Sarah at [email protected] for more information.

If you are interested in any of rtilab's products, please fill out our request form below and we will get an invoice emailed to you as soon as we can. 

DOMESTIC ORDERS

  • Payment by credit card, over the phone or via email, and by electronic bank wire accepted.
    • A 3.5% credit card fee will apply.
  • PO numbers are accepted from established clients.
  • Shipping and handling rates depend on where you are located, orders may vary.
    • You are more than welcome to use your own FedEx account number.
    • We ship domestic orders FedEx Priority Overnight.
    • $25 handling fee will apply.
  • We ONLY ship domestic orders on Monday and Tuesday of each week.


INTERNATIONAL ORDERS

  • Payment is required BEFORE we ship your product.
  • Payment only by electronic bank wire will be accepted.
    • Additional $35 fee for international wire payment applies.
  • ALL shipping costs are at the expense of the purchaser. 
    • YOUR FedEx account number is REQUIRED prior to shipment.
    • We ship international orders FedEx Priority.
    • Products are shipped on dry ice, which is included in a $90 handling fee.
    • Our shipping box dimensions are 16x16x16 and weigh about 17 lbs. 15lbs of which is dry ice.
  • We ONLY ship international orders on Monday of each week.
     

PAYMENT TERMS AND PRODUCT CONDITION SHIPMENT WARRANTIES

Payments: All products must be paid for prior to shipment by credit card or money transfer. 

Shipping Warranties: rtilab warrants the condition of its products and the proper packaging at the time of transfer to the designated shipper. We do not warrant the “product condition” from shipment or customs delays or errors, and for any receiving delays. Once the product is received by the shipper rtilab is no longer responsible for the package and its contents. This applies to both domestic or international shipments. The customer is responsible for meeting all customs and other regulatory requirements. We will package the product for shipment as requested on the customer order form. We will schedule shipment with the desired carrier for the desired delivery time and desired rate. Customer must indicate if they wish for shipping insurance to be included. We welcome the domestic customer to make their own shipment arrangements and we require that international customers make the shipping arrangements and schedule a pick-up date and time with us.