Bovine Coronavirus (BCV) Monoclonal Antibody (BC 6-4) ♦ BC 6-4 reacts with North American strains of Bovine Coronavirus (BCV). This monoclonal antibody recognizes an epitope on the nucleocapsid protein of BCV. The antibody is produced as mouse ascites fluid. One BC 6-4 product is available: BC 6-4-A (unconjugated ascites fluid). Isotype-IgG1. Uses of BC 6-4-IHC, IFA, ELISA
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Monoclonal Antibody (AT13-A) ♦ Monoclonal antibody AT-13 recognizes a highly conserved, conformational dependent epitope within the nucleocapsid protein of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). It was produced against a European-like strain (U.S. type I) recently isolated within the United States. It has a high degree of cross reactivity to European, European like, and North American isolates of PRRSV. This monoclonal antibody is produced as mouse ascites fluid. One AT13 product is available: AT13-A (unconjugated ascites fluid). Isotype: IgM. Product AT-13 is recommended for use in immunoprecipitation, indirect fluorescent antibody, and enzyme linked-immunosorbent assay applications. AT-13 does not recognize proteins on Western blot.
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Monoclonal Antibody (SDOW17) ♦ SDOW17 reacts with >99% of North American and European isolates of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). This monoclonal antibody recognizes a highly conserved epitope on the nucleocapsid protein of PRRSV. SDOW17 does not react with the Prime Pac PRRSV vaccine strain. This antibody is produced as mouse ascites fluid. Two SDOW17 products are available: SDOW17-A (unconjugated ascites fluid) and SDOW17-F (ascites fluid conjugated with fluorescein isothiocyanate {FITC}). Isotype-IgG1. Uses and Recommended Dilutions - SDOW17-A: IFA—1:50,000, IHC—1:10,000. SDOW17-F: IFA—1:100
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Monoclonal Antibody (SR30) ♦ SR30 reacts with essentially all North American and European strains of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). This monoclonal antibody recognizes a highly conserved epitope on the nucleocapsid protein of PRRSV. The antibody is produced as mouse ascites fluid. Two SR30 products are available: SR30-A (unconjugated ascites fluid) and SR30-F (ascites fluid conjugated with fluorescein isothiocyanate {FITC}). Isotype-IgG1. Uses and Recommended Dilutions - SR30-A: IFA—1:50,000, IHC—1:10,000, SR30-F: IFA—1:100
Escherichia coli K88ac Monoclonal Antibody (EC K88) ♦ The anti-K88ac monoclonal antibody was created against the K88 fimbriae of enterotoxigenic Escherichia coli (ETEC). The antibody reacts with K88 fimbriae of ETEC and blocks the binding of K88+ bacteria to intestinal epithelial cells. The antibody is produced in hybridoma cells and is available as cell culture supernatant. Isotype-IgG1. Uses of EC K88-IFA. Recommended Dilution—1:100
Rotavirus (RV) Monoclonal Antibody (RV 11-2) ♦ RV 11-2 reacts with Group A strains of rotavirus (RV). This monoclonal antibody recognizes an epitope on the VP6 protein of RV. The antibody is produced as mouse ascites fluid. One RV 11-2 product is available: RV 11-2-A (unconjugated ascites fluid). Isotype-IgG2a. Uses of RV 11-2: RV11-2-A: IFA, ELISA.
Porcine Circovirus Monoclonal Antibody (PCV2) ♦ PCV2 reacts with porcine circovirus 2 virus. The antibody is produced as mouse ascites fluid. One PCV2 product is available: PCV2-A (unconjugated ascites fluid). Isotype-IgG2. Uses and Recommended Dilution, IFA—1:1000
Antibodies can be conjugated to different labels, including enzymes, biotin, and fluorescent dyes/proteins. The label of choice essentially depends upon the experimental application.
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light, causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue, or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques because the conjugation of FITC to proteins is relatively easy and generally does not destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Antibodies to FITC are used to identify FITC labeled proteins and as models to study the mechanism of antibody response to a well-defined hapten.
Biotin is commonly used when the target of interest is expressed at low levels and cannot be detected using secondary labeled antibodies alone. Many biotin molecules can be conjugated to a secondary antibody.
Enzymes are proteins, and the molecules upon which they act are known as substrates. The enzyme label can be visualized by means of enzyme histochemical methods via chromogenic reactions, wherein a soluble colorless substrate is converted to a water-insoluble colored compound. One of the more commonly used enzymes is Horse radish peroxidase (HRP).
Horse radish peroxidase (HRP) can be visualized by chromogenic reactions such as chemiluminescence or through the use of diaminobenzidine (DAB). HRP is a 44kDa glycoprotein enzyme label and is more stable than alkaline phosphatase.
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